[Histonet] TUNEL
Hi Everyone,
I am going to begin some TUNEL experiments for the first time and have
some basic questions. First, how difficult is it to stain un-sectioned
embryonic tissue? The target cells are about 4-5 cell layers in from
the surface. Second, the target cells are transgenically labeled with
YFP--will the YFP signal,or its epitope, be lost by the tissue prep for
TUNEL? Lastly, could I get some perspective on good/bad TUNEL kits? I
have found some of this topic in the archive, but this advice may have a
shelf life. Thanks in advance.
Cordially,
Noah Druckenbrod
University of Wisconsin-Madison
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