Brian,
never did this on cells but used to use VK to measure calcified area in bone
biopsies allt the time.  We used an image analysis system with a video
camera on a scope and would measure the total area in a field (usually 10x)
and then measure the black calcified area which got reported out as "total
area of sample calcified".  not sure how you could do this on cells.  with
bone we had standards for different components and they stayed in place in
relation to each other.  we knew given a sample size how much of it should
be cortical bone, trabecular bone, marrow, fat, etc.  i suppose if you know
the normal area of your cells when they are not calcified you could apply
something like this.
I would refer you to a paper we wrote An Indirect Method of Measuring Widths
Suitable for Automated Bone Histomorphometry  Journal of Bone and Mineral
Research  Vol. 7 NO. 12, 1992


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of Brian
Hatcher
Sent: Tuesday, January 20, 2004 8:33 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Quantitative von Kossa


Is anyone aware of a technique/references on extracting quantitative
information from a von Kossa stain.  I am interested in trying to measure
the rate and extent of mineralization in a marrow stromal cell culture, and
would like to know if this is possible using this stain, and also what
target values I should be trying to achieve.
Thanks.

Brian Hatcher
Graduate Research Asst.
Dept. of Biomedical Engineering
University of Florida


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