double label IHC stains for skin biopsies

From:

Dmborel@aol.com

Does anyone know of a lab which can perform double label IHC stains involving a different chromagen for each of the two labels: CD4+CD45RO+ (UCHL-1) and CD8+CD45RO+ (UCHL-1) on formalin fixed paraffin embedded tissue or on frozen tissue? Is there a way to quantitate these, or must one do a semiquantitative examination by estimating the percentage of positive cells?

Thanks for your help. These have been requested as part of a research project for a drug trial.

David M. Borel, MD
Dermatopathology Diagnostics

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