Hi histonetters,

Another factor to bear in mind when cutting large volumes of control slides 
is that the site of interest might be only localised and that it might 
disappear from the section. This is not of crucial importance when using  
normal tissue, but is when using tumour blocks.
Just my 2 cents worth!


Louise Renton
Bone Research Unit
MRC
Johannesburg
South Africa
Tel & fax +27 11 717 2298
"Time flies like an arrow, fruit flies like a banana"





>From: DDittus787@aol.com
>To: clarke.ian@virgin.net, histonet@pathology.swmed.edu
>Subject: Re: Re-Immuno control sections
>Date: Wed, 29 Jan 2003 14:18:50 -0500
>
>Ian:
>In the past I have cut  nuclear antigen control slides(er,pr ki-67) and 
>frozen them at -80(unbaked) i bake after they defrosted and had them keep 
>antigenicity for a good 6 months, also i take other controls like 
>cytokeratins ((which lose antigenicity at a slower rate) and cut and store 
>at 4 degrees. because of these observations i now store all control slides 
>(s100, hmb45, vimentin , etc. at 4 degrees and usually only cut about 2 
>dozen in advance. hope this helps.
>                           dana


_________________________________________________________________
Get free web space now! http://groups.msn.com/home