Thin sections (was Microtomes)

From:"Morken, Tim"

That's very tough. And what would you gain? Too thin and you lose contrast
to the point you won't see anything. What, exactly is the specimen and what
is the desired outcome?

Tim Morken

-----Original Message-----
From: Ephram Shizgal []
Sent: Wednesday, January 29, 2003 8:54 PM
To: 'HistoNet Server'

I have been interested in tossing a general microtome/sectioning question
out to the list and this seems like a good place for it to go:

If someone were to suggest that you needed to produce sections between 20
and 30 nm to use on a specific TEM to get results, is this a thickness that
most of you feel is attainable (albeit something of a challenge) or, in
fact, too challenging to be routinely considered ?

Thanks in advance for the feedback.


Ephram Shizgal
Delong America (a member of the LVEM5 Group of Delong Instruments)
In USA and Canada: 1-866-DELONGUSA (1-866-335-6648)
International: 514-904-1202

For LVEM5 (Low Voltage Electron Microscope)
information please visit 

-----Original Message-----
From: Georger, Mary [] 
Sent: Wednesday, January 29, 2003 7:45 PM
To: HistoNet Server; 'Timothy R. Wheelock'

Hi Tim,
I have dhad the Microm HM 355 S for several years and asolutely love it! We
do many very tedious serial cuts of entire vessels, and the machine makes my
life so much easier. It is reliable, easy to learn, and thus far I have had
no problems. 
Best of luck!
Mary Georger
Center for Cardiovascular Research
University of Rochester Medical Center
KMRB Room 2-9816
601 Elmwood Avenue.
PO Box 679
Rochester, New York  14642

> ----------
> From: 	Timothy R. Wheelock
> Sent: 	Wednesday, January 29, 2003 4:30 PM
> To: 	HistoNet Server
> Subject: 	MICROTOMES
>  Hi Everyone:
> I am in the market for a microtome.
> I have tested 3 fully automated machines and 3 manual machines. The 
> electric microtomes are the Richard Allan Microm HM 355 S, the Shandon  
> Finesse ME, and the Leica RM 2155. I did reference checks on all 3 
> electric machines.
> I eliminated the manual machines (by the same 3 companies) mainly
> because I found their flywheels much harder to turn than my present 30 
> year old AO820!
> I am leaning toward 1 of the electric microtomes, but thought I would
> ask people's opinion.
> One of the many issues  i am concerned about is durability, workmanship, 
> whether a machine is solidly built.
> Thanks for your time,
> Tim Wheelock
> Harvard Brain Tissue Resource Center (Brain Bank)
> McLean Hospital
> Belmont, MA

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