Please post replies to Histonet. Thanks, Marge

-----Original Message-----
From:	Dmborel@aol.com [SMTP:Dmborel@aol.com]
Sent:	Wednesday, January 29, 2003 2:59 PM
To:	histonet@pathology.swmed.edu
Subject:	double label IHC stains for skin biopsies

Does anyone know of a lab which can perform double label IHC stains involving a
different chromagen for each of the two labels: CD4+CD45RO+ (UCHL-1) and
CD8+CD45RO+ (UCHL-1) on formalin fixed paraffin embedded tissue or on frozen
tissue?  Is there a way to quantitate these, or must one do a semiquantitative
examination by estimating the percentage of positive cells?

Thanks for your help.  These have been requested as part of a research project
for a drug trial.

David M. Borel, MD
Dermatopathology Diagnostics