RE: double label IHC stains for skin biopsies
Please post replies to Histonet. Thanks, Marge
From: Dmborel@aol.com [SMTP:Dmborel@aol.com]
Sent: Wednesday, January 29, 2003 2:59 PM
Subject: double label IHC stains for skin biopsies
Does anyone know of a lab which can perform double label IHC stains involving a
different chromagen for each of the two labels: CD4+CD45RO+ (UCHL-1) and
CD8+CD45RO+ (UCHL-1) on formalin fixed paraffin embedded tissue or on frozen
tissue? Is there a way to quantitate these, or must one do a semiquantitative
examination by estimating the percentage of positive cells?
Thanks for your help. These have been requested as part of a research project
for a drug trial.
David M. Borel, MD
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