Michaela:
How thick are your sections?  My experience using any
of the methacrylates (including the JB-4 kits) has
been good using all of the routine stains, including
H&E.  I have gotten excellent results on 1 and 2um
thick sections.

Roger Moretz, Ph.D.
Dept. of Toxicology
BI Pharmaceuticals, Inc.

--- Michaela_Fr#253#zkov#225# 
wrote:
> Dear Colleagues,
> I need an advice about staining sections embedded in
> JB-4 Plus. I embed in
> this medium and now I need stain my sections. I
> wanted to use regular
> haematoxylin-eosin staining, but it doesn#180#t
> work. Do you know something
> about that? Can you recommend me some other
> staining?
> 
> Thank you, very much.
> Michaela Fryzkova
> 
> Department of Parasitology
> Faculty of Science
> Charles University
> Prague
> Czech Republic
> 
> 


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