Re: Masson Trichrome stain
I am not familial with the commercial kits but going
on the assumption that they are similar to the 'homemade' stain, two possibilities
come to mind.
1. Inadequate fixation resulting in poor nuclear staining by hematoxylin.
2. Nuclear staining by Harris heamtoxylin is being 'bleached' by subsequent
(acidic) steps. To remedy this treat sections with a 1:1 mixture of 2.5%
phosphomolybdic acid : 2.5% phosophotungstic acid for 30-60 seconds. This
treatment is applied after hematoxylin staining is finished (and differentiated)
but before subsequent proceedures. Then wash and proceed to remaining steps.
See Katline, VC. Stain Technology 37:193-195, 1962.
Geoff
"Demarinis,Carolyn" wrote:
Each time we perform a Masson Trichrome stain, the
nuclei are bright red. I
have used Polyscientific and Sigma kits, with both Weigerts and Harris
Hematoxylin. Can anyone offer any suggestions?
--
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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