RE: CD3 (poly)

From:"Dawson, Glen"

I would concur with Robert on this antibody.  It is a good one.

Glen Dawson

-----Original Message-----
From: Lott, Robert []
Sent: Monday, January 27, 2003 3:59 PM
Subject: CD3 (poly)

Cell Marque has a polyclonal equivalent to the DAKO product.  When we found
out about the DAKO backorder, we bought the Cell Marque antibody and now use
it at 1:1000, steam in Citrate buffer for 15 min., with Powervision+
detection.  It looks great!

The literature will suggest that the monoclonal CD3(clone PS1) is a very
good antibody, perhaps a wee bit more specific than the polyclonal
antibody... it has been my experience however that it must be used at a much
higher dilution (around 1:40-1:50), making the polyclonal far more

Robert L. Lott, HTL(ASCP)
      Manager, Anatomic Pathology
      Baptist Health System
      800 Montclair Road
      Birmingham, AL  35213
      205-592-5646 (fax)

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