| From: | Tony Henwood |
One method:
Place drop of blood (no anti-coagulanr added-1cm in diam) onto slide, allow to clot (5min, place in 10% formalin to fix, scrape off with a blade onto processing paper, place in cassette and process as usual.
Or:
Centrifuge blood, remove supernatant, add 2-3 drops of thromboplastin (from haematology), 2-3 drops plasma, 2-3 drops 1% calcium chloride, mix and let stand for 5 min to let clot form. Add formalin and process as usual.
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318
http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html
-----Original Message-----
From: Margarete Vermehren
[mailto:m.vermehren@anat.vetmed.uni-muenchen.de]
Sent: Friday, 17 January 2003 18:55
To: histonet@pathology.swmed.edu
Subject: clot preparations of peripheral blood
sorry, I#180#ve forgot to add a subject to my last e-mail!!
Margarete Vermehren
LMU M#252#nchen / Tieranatomie II
Tel: +49 89 21805857
Fax: +49 89 21802569
m.vermehren@anat.vetmed.uni-muenchen.de
-----Urspr#252#ngliche Nachricht-----
Von: Margarete Vermehren [mailto:m.vermehren@anat.vetmed.uni-muenchen.de]
Gesendet: Friday, January 17, 2003 8:51 AM
An: 'histonet@pathology.swmed.edu'
Betreff:
hello,
does anybody know a protocoll for preparation of blood clot sections.
I`ve read several times about formalin-fixed paraffin-embedded blood clots
without any description of the protocol.
can somebody please help?!
Thanks a lot!!
Margarete Vermehren
LMU M#252#nchen / Tieranatomie II
Tel: +49 89 21805857
Fax: +49 89 21802569
m.vermehren@anat.vetmed.uni-muenchen.de
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