LM: freezing tissue - method?

From:Tamara Howard

I'm sort of conducting a survey - how do you freeze blocks prior to
cryostat sectioning? A PI in our department is writing a grant and offered
to include a freezing set-up (we just use a dewar of LN2 now) - the only
equipment I've come across on the web is the giant NesLab bath. Is a
styrofoam cooler of LN2 with a beaker of some solvent still a reasonable
way to go, or is there some spiffy gizmo we can buy?

Thanks! (Sorry about the cross-post for those of you on both lists)


 Tamara Howard
 Department of Cell Biology and Physiology
 University of New Mexico - Health Sciences Center
 Albuquerque, NM 87131

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