Only got a sample of Cell Marque's predilute CD3. In our hands, it wasn't as
good as Dako's predilute polyclonal (cat N1580)

Ronnie Houston
Regional Histology Operations Manager
Bon Secours HealthPartners Laboratories
5801 Bremo Road
Richmond,VA 23226

-----Original Message-----
From: Dawson, Glen [mailto:GDawson@Milw.Dynacare.com]
Sent: Tuesday, January 28, 2003 10:34 AM
To: Lott, Robert; histonet@pathology.swmed.edu
Subject: RE: CD3 (poly)


I would concur with Robert on this antibody.  It is a good one.

Glen Dawson

-----Original Message-----
From: Lott, Robert [mailto:Robert.Lott@bhsala.com]
Sent: Monday, January 27, 2003 3:59 PM
To: histonet@pathology.swmed.edu
Subject: CD3 (poly)


Cell Marque has a polyclonal equivalent to the DAKO product.  When we found
out about the DAKO backorder, we bought the Cell Marque antibody and now use
it at 1:1000, steam in Citrate buffer for 15 min., with Powervision+
detection.  It looks great!

The literature will suggest that the monoclonal CD3(clone PS1) is a very
good antibody, perhaps a wee bit more specific than the polyclonal
antibody... it has been my experience however that it must be used at a much
higher dilution (around 1:40-1:50), making the polyclonal far more
economical.

Robert L. Lott, HTL(ASCP)
      Manager, Anatomic Pathology
      Baptist Health System
      800 Montclair Road
      Birmingham, AL  35213
      205-592-5388
      205-592-5646 (fax)
      robert.lott@bhsala.com