Fwd: Re: Trichrome staining.
Russ
is right here. The celestine blue I use is Lendrum, A.C. 1935. J. Path.
Bact. 40. 415-416. Celestine Blue as a nuclear stain.
Ian.
Date: Thu, 30 Jan 2003 09:30:41
+0000 (GMT0BST)
From: RUSS ALLISON <Allison@Cardiff.ac.uk>
Subject: Re: Trichrome staining.
To: "J. A. Kiernan" <jkiernan@uwo.ca>
Cc: histonet@pathology.swmed.edu
I have a feeling that Lendrum was involved in using celestine blue /
haemalum sequence, but, like you, have been unable to reference
it.
Unfortunately, Bill Slidders, who worked with Lendrum more latterly,
has now retired and is not very well, I understand.
As an old friend, I might otherwise been able to call him.
Date sent:
Thu, 30
Jan 2003 00:33:18 -0500
From:
"J.
A. Kiernan" <jkiernan@uwo.ca>
Subject:
Re:
Trichrome staining.
To:
Tony
Henwood <AnthonyH@chw.edu.au>
Copies to:
'Ian
Montgomery' <ian.montgomery@bio.gla.ac.uk>,
histonet@pathology.swmed.edu
Tony raises an interesting point. I haven't been able
to find the original publication of iron-celestine
blue followed by haemalum, which has been popular
for many years in the English-speaking world outside
North America. There are plenty of published references
but all the ones I've seen cite secondary sources.
Even the superb 796-page fifth edition of "The
Theory and Practice of Histological Techniques"
(Bancroft & Gamble, 2002) gives a procedure with
no references or even speculation about how the
method works. There are plenty of old papers about
iron-celestine blue B alone as a nuclear stain. Why
is it advantageous to follow it up with a haemalum?
--
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan@uwo.ca
http://publish.uwo.ca/~jkiernan/
_________________________________________
Tony Henwood:
> Remember that Celestine Blue is made up in 5% iron alum (ferric
ammonium sulphate). So is its stability, in the face of the
trichrome
> acidic stains, due to an iron mordant rather than the celestine blue
dye itself. ?very much like an Iron Haematoxylin stain like
> Weigert's!!
>
> I like the Celestine blue/Haematoxylin sequence as well.
>
> Just a thought!!
>
>
> Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager
> The Children's Hospital at Westmead,
> Locked Bag 4001, Westmead, 2145, AUSTRALIA.
> -----Original Message-----
> From: Ian Montgomery
[mailto:ian.montgomery@bio.gla.ac.uk]
> Sent: Thursday, 30 January 2003 1:59
> To: histonet@pathology.swmed.edu
> Subject: Trichrome staining.
Ian Montgomery:
> Although a well executed Weigert is superb
for a Masson I still stand by my
> Celestine blue/Haematoxylin sequence. Over
the years I've used it repeatedly and always
> get beautiful staining. If like me your in
the process of staining ~500 slides with a Masson
> then the ease and reproducibility of the
Celestine blue/Haematoxylin sequence has a lot to
> commend it.
> Ian.
>
> Dr. Ian Montgomery,
> Histotechnology,
> Graham Kerr Building,
> Institute of Biomedical & Life
Sciences,
> University of Glasgow,
_______________________________________________________________
Russ Allison,
Dental School
Cardiff
Wales
Dr. Ian Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery@bio.gla.ac.uk
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