Dear Wendy,

Our lab has had good luck with the Histobath.   It is
easy to maintain and produces better specimens than
when snap freezing in liquid nitrogen.  The only
drawback I have noticed is the cost  of  using
isopentane, as it needs to be changed frequently or it
will pick up water over time and not cool to the
desired temperature as quickly.

Liz Lummus
Biochemistry Dept.
UT Southwestern Medical Center Dallas


-- "wprime@liverpool.ac.uk" 
wrote:
>  Dear Histonetters 
> 
> I am thinking of purchasing a Shandon Histbath  to
> freeze 
> tissue.  I would welcome comments from any body who
> has or 
> is currently using one. 
> 
> As a Tissue Bank  our remit is to freeze tissue  for
> 
> research projects.
> 
>  
> Regards
> 
> Wendy
> 
> ----------------------
> wprime@liverpool.ac.uk
> Mrs Wendy Prime 
> CTBRC 
> Department of Pathology
> University of Liverpool
> Tel: 0151 706 4503
> Fax: 0151 706 5859
> 
> 
> 
> 


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