Mary,
We also use Lithium carbonate to blue the Hx in HE
sections. We dilute a saturated aqueous Lithium
Carbonate solution 1/10 in water. Other blueing
solutions, of course include:
Scott's Tap water substitute
Ammonia in water
Warm running tap water
Any slightly alkaline solution will do.


 --- "Cohen, Mary Ann"  wrote:
> 
>   Hi all,
> 
> My question is,  for an H&E stain when making up
> your lithium carbonate
> solution after your Hematox dip, I am using a
> saturated aqueous lithium
> carbonate solution with 1 gram of lithium into 100
> ml of  ( this is the
> question ) do I use distilled H2O, or 100 % EtOH? 
> If my memory serves me
> after many years of working in the lab and smelling
> all of the chemicals,  I
> would use EtOH,   is this correct.
> 
> I would appreciate your comments.
> 
> Food for thought,  I do not want to use pre-made
> clarifier.
> 
> Thanks,
> 
> MaryAnn Cohen
> Pathology Assistant
> MTS II
> BioReliance
> 9630 Medical Center Drive
> Rockville, MD 20850-3349
> 301-610-2192
> mcohen@bioreliance.com
>  
> fax # 301-838-0371
> 
>  

=====
Tony Henwood JP BAppSc GradDipSysAnaly CT(ASC)
Laboratory Manager
Histopathology Department
The Childrens Hospital at Westmead
Locked Bag 4001
WESTMEAD, 2145
AUSTRALIA

http://my.yahoo.com.au - My Yahoo!
- It's My Yahoo! Get your own!