Re: lithium carbonate
We also use Lithium carbonate to blue the Hx in HE
sections. We dilute a saturated aqueous Lithium
Carbonate solution 1/10 in water. Other blueing
solutions, of course include:
Scott's Tap water substitute
Ammonia in water
Warm running tap water
Any slightly alkaline solution will do.
--- "Cohen, Mary Ann" wrote:
> Hi all,
> My question is, for an H&E stain when making up
> your lithium carbonate
> solution after your Hematox dip, I am using a
> saturated aqueous lithium
> carbonate solution with 1 gram of lithium into 100
> ml of ( this is the
> question ) do I use distilled H2O, or 100 % EtOH?
> If my memory serves me
> after many years of working in the lab and smelling
> all of the chemicals, I
> would use EtOH, is this correct.
> I would appreciate your comments.
> Food for thought, I do not want to use pre-made
> MaryAnn Cohen
> Pathology Assistant
> MTS II
> 9630 Medical Center Drive
> Rockville, MD 20850-3349
> fax # 301-838-0371
Tony Henwood JP BAppSc GradDipSysAnaly CT(ASC)
The Childrens Hospital at Westmead
Locked Bag 4001
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