Hi Dina,
Here's the Oil Red O that I've used, but should be used on frozen sections
because regular processing disintegrates the fat.

Oil Red O
Fixative:    None
Technique:  Cut frozen sections at 4 to 6 micronsl.
Quality Control:  Tissue containing fat.

Staining Procedure
1.  Stain slides in working Oil Red O for 10-15 min
2.  Wash in tap water.
3.  Mayer's Hematoxylin for 10-30 sec.
4.  Wash rinning water for 3-5 min
5.  Mount with water based mounting media.

Results
     Fat...........................red
     Nuclei.....................blue

Solutions
     Oil Red O (stock)
        Oil Red O.......................0.5 gm
        Isopropyl Alcohol...........100 ml

     Oil Red O (working)
         Oil REd O (stock)...............6.0 ml
         Distilled water.....................4.0 ml
         mix just before use and let stand 7-10 min, filter

Reference
        Lillie, R.D.; 1954.  "Histopathologic Technic and Practical
Histochemistry".  (2nd Ed.)  (pp. 303)


Nancy Weber
Research Associate
Veterinary Clinical Sciences
Ohio State University
----- Original Message -----
From: 
To: 
Sent: Monday, January 28, 2002 7:21 PM
Subject: Oil Red O


> Hello Histonetters,
>
> Does anyone have a protocol for staining ORO on paraffin sections?
>
> Thanks,
>
> Dina
>
>