I don't want to get in to the use of zinc formalin, but my personnal opinion is that it is dangerous to use  blanket antigen retrieval for every antibody.
It simply is not necessary for all antibodies, and indeed enhances non-specific staining.

Ronnie Houston
Cytochemistry & Molecular Pathology
Texas Scottish Rite Hospital
2222 Welborn Street
Dallas, TX 75219
USA
(214) 559 7744
(214) 559 7768 - fax
ronnie.houston@tsrh.org

>>> Laurie Colbert  01/25/02 11:04AM >>>
I was wondering if it was necessary to use zinc formalin to enhance IHC
staining and prevent cross-linking if you were doing antigen retrieval
for every antibody.  Any comments???

Laurie Colbert