Re: Antigen retrieval
Laurie,
We used zinc formalin because it also enhanced the
nuclear staining on our routine H&E staining. We did a parallel study
with formalin and with a zinc isopropyl formalin (Carson et al, JOH). All
of our tissues received a secondary fixation of the zinc isopropyl solution on
the processor. Many of our antibodies were enhanced, but we still needed
to do antigen retrieval on some. Will all of your tissues be fixed in zinc
formalin? Remember also that if you go back to tissues that were not fixed
in the zinc formalin you may obtain different staining reactions
Have you tested all of your antibodies with and
without antigen retrieval. We found that some antigens or antibodies did
not survive the extreme treatment of antigen retrieval. Many of the
antibody/antigen reactions were much more intense without the antigen
retrieval.
Jennifer MacDonald
I
was wondering if it was necessary to use zinc formalin to enhance IHC staining
and prevent cross-linking if you were doing antigen retrieval for every
antibody. Any comments???
Laurie Colbert
<< Previous Message | Next Message >>