This email came to me and I know this has been discussed before so maybe 
somebody can help her out.
Thanks.
Andi



>Date: Thu, 31 Jan 2002 17:57:58 +0000
>From: Joan Edwards 
>Subject: Frozen Section Histology for Collagen?
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>
>Hi,
>
>My name is Joan Edwards and I am a PhD student at the Rowett Research 
>Institute in Aberdeen, Scotland.
>
>I am trying to find out how to go about staining rumen tissue samples for 
>collagen.  I would prefer to stain frozen sections which I plan to do 
>in-situ hybridisation work with but all the literature I find talks of 
>fixed tissue or paraffin embedded.  Do you know of a method that is 
>suitable for collagen staining of frozen sections?  And should I do this 
>before or after in-situ study of the same section - if indeed I can do the 
>staining on the same section?
>
>Any advice would be very gratefully appreciated.
>
>Regards
>
>Joan
>

.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
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