My motto has always been, I have never met a blade that is too sharp!

 Dulling a c profile steel knife seems counterproductive -  however, using
the cryomatrix is the better way to prevent nicks, etc that create ski
tracks and it seems that after you run a few cuts with cryoembedding media,
you ARE getting better cuts.  It may be that you have removed any oil
coating the edge after reconditioning with the frozen cryomatrix.  Residual
oil can be cleaned off edge with 100% alcohol or acetone.  

When a blade is resharpened/reconditioned it changes blade to some degree
and the antiroll device would have to be carefully checked for readjustment
to compensate. OR you could try adjusting your knife angle a tidge to make
sure the sections come off flat.  Knife angle may be one important factor.

Personally, I think the oil is your problem

Happy cutting

  

   

At 11:30 AM 1/10/02 -0500, you wrote:
>i use a cryostat to section my embryos and every so often the blade gets 
>sent out to be sharpened.  when we get the blade back it is SO sharp that 
>the tissue immediately curls before we can get it on a slide.  we do use an 
>anti-roll plate.  recently we have been using old embryos or straight frozen 
>cryo matrix to dull the blade enough to stop the curling.  this however, 
>takes a while.  does anyone have any suggestions on how to dull the blade 
>once it's freshly sharpened?
>-alison-
>
>
>
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Gayle Callis
MT,HT,HTL(ASCP)
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)