Fred,
That is a great idea.  I use the fluorescence of fresh eosin to demonstrate
osteoid on a Von Kossa stained mineralized bone section and it works great, the
calcified bone is dark from the silver stain and the osteoid lights up under UV
like a christmas tree.
Patsy Ruegg

"Monson, Frederick C." wrote:

> Morning,
>         Just on the chance that this would be appropriate for your effort, I
> will make a suggestion for a simple, not quite-non-destructive, method for
> spotting early capillary damage in routine specimens.
>         Background:  Fresh Eosin preparations have a green sheen when
> exposed to the UV in daylight or even fluorescent illumination.  FRESH!
>
>         Utility:  As a consequence of the eosinophilia of RBC's, one can use
> any fluorescent scope with a FITC cube or filters to demonstrate the
> distribution of RBC's in any H&E section.
>
>         Downside:  After exposure to UV the eosin background will have a
> visible hole in it when viewed with normal brightfield illumination.
>
>         Upside of Downside:  One can always relocate those areas on which
> one paused 'for a look' while using the UV.
>
> Hope this helps,
>
> Fred Monson
>
> Frederick C. Monson, PhD
> Center for Advanced Scientific Imaging
> West Chester University
> West Chester, Pennsylvania, USA, 19383
> 610-738-0437
> fmonson@wcupa.edu
>
> > ----------
> > From:         Zubovits, Dr. Judit
> > Sent:         Wednesday, January 9, 2002 7:19 PM
> > To:   'histonet@pathology.swmed.edu'
> > Subject:      vital stain
> >
> > Hello Histonet-ters;
> >
> > I wonder if you could help me with the following:
> >
> > I am looking at "muscle biopsies" from volunteers (mice) who have received
> > focused ultrasound treatment.  Of course enough excess heat would cook any
> > tissue, but I we are trying to detect much more subtle changes/damage
> > produced by a lot less heat.
> >
> > I know TTC has been used on hearts to detect ischemic necrosis associated
> > with myocardial infarction, but as I understand it, you need to soak the
> > tissue *before* paraffin embedding, and TTC is intended to show areas of
> > necrosis at the gross, not microscopic level.
> >
> > Is there anything out there for the microscopic level that would highlight
> > areas of damage?
> >
> > Thanks for all your help.
> >
> > Judit Zubovits
> >
> >