Mike,
You are right, we saw langerhan cells picking up staining with many different
antibodies.
Patsy

Michael Archambault wrote:

> It is quite possible that you have picked up the Langerhans cells in the
> epidermis- those rogue wandering dendritic cells that are part of the
> immunologic protective function in the epidermis (including gingiva).  I
> don't recall offhand if the langerhans cells express CD3 constituatively,
> but I'm sure there are plenty of situations where CD3 may be upregulated.
> Also remember that you may be picking up T cell staining within the arms of
> the dendritic cells, which may be difficult to differentiate from the
> dendritic cells themselves.  If you're investigating these cells, there are
> mouse strains that are immunocompromised in various capacities, and specific
> strains are available that lack dendritic cells and langerhans cells.
>
> Hope this helps!
>
> -Mike
>
> Michael Archambault, Research Scientist
> Bone and Soft Tissue Program
> Osiris Therapeutics Inc.
> 2001 Aliceanna St  Baltimore, MD 21231
> 410 522-5005 x 226
> marchambault@osiristx.com
>
> -----Original Message-----
> From: Cristiano Rumio [mailto:cristiano.rumio@unimi.it]
> Sent: Tuesday, January 08, 2002 2:52 AM
> To: histonet@pathology.swmed.edu
> Subject: CD 3
>
> Dear collegues,
>
> I used CD3 Antibody from DAKO on mouse skin. I was surprised when I found
> dendritic cells stain in the epidermis. HAve you an idea of which kind of
> cells they are? I
> In my opinion this antibody does not work well, have you the some
> experience?
>
> Thank you in advance.
> Cristiano Rumio
>
> Prof. Cristiano Rumio
> Department oj Human Anatomy
> Via Mangiagalli 31
> 20133 Milan
> Italy
> Tel. +39-0258355401
> Fax. +39-022364082
> e-mail cristiano.rumio@unimi.it