Mary,
I switched to Santa Cruz monoclonal VEGF with good results on rats.
Patsy

"Georger, Mary" wrote:

> Andrea,
> I use Santa Cruz SC-507 at 1:200 overnight at 4C. Pretreat with DAKO
> digestion for 6 minutes, use biotinylated secondary, Vector ABC and DAB. The
> system is polyclonal, I have it working well on mouse tissues but I would
> love to know what monoclonal you are using. We use poly because that is what
> the researcher had already purchased long ago and far away.
> Have a great weekend!
>
> Mary Georger
> Center for Cardiovascular Research
> University of Rochester Medical Center
> KMRB Room 2-9816
> 601 Elmwood Avenue.
> PO Box 679
> Rochester, New York  14642
> 585-273-1548
>
> > ----------
> > From:         AndreaH@imclone.com
> > Sent:         Thursday, January 10, 2002 8:12 PM
> > To:   histonet@pathology.swmed.edu
> > Subject:      anti-mouse VEGF
> >
> > Does anyone have a strong protocol for anti-mouse VEGF staining preferably
> > of paraffin sections? I am optimizing my protocol and would like to know
> > what protocol works well for everyone else.
> >
> > Thank you in advance.
> > Andrea
> > andreah@imclone.com
> >
> >
> >