Sorry about missing part of the point, but here is another possibility.

	Evans blue is commonly used as a vital dye to 'find' points of
extravasation of blood.  In this case, the Evans blue, when injected i.v.
binds preferentially with the blood albumin.  In this combination, the
albumin-Evans blue complex is fluorescent. 

	If you're interested in this let me know.

Regards,

Fred Monson 

> ----------
> From: 	Zubovits, Dr. Judit
> Sent: 	Wednesday, January 9, 2002 7:19 PM
> To: 	'histonet@pathology.swmed.edu'
> Subject: 	vital stain
> 
> Hello Histonet-ters;
> 
> I wonder if you could help me with the following:
> 
> I am looking at "muscle biopsies" from volunteers (mice) who have received
> focused ultrasound treatment.  Of course enough excess heat would cook any
> tissue, but I we are trying to detect much more subtle changes/damage
> produced by a lot less heat.  
> 
> I know TTC has been used on hearts to detect ischemic necrosis associated
> with myocardial infarction, but as I understand it, you need to soak the
> tissue *before* paraffin embedding, and TTC is intended to show areas of
> necrosis at the gross, not microscopic level.
> 
> Is there anything out there for the microscopic level that would highlight
> areas of damage?
> 
> Thanks for all your help.
> 
> Judit Zubovits
> 
>