Is anyone still using buffer and not serum for negative controls? Thanks, j
Joyce Weems
Pathology Manager
Saint Joseph's Hospital of Atlanta


	-----Original Message-----
	From:	Gervaip@aol.com [SMTP:Gervaip@aol.com]
	Sent:	Wednesday, January 31, 2001 10:15 PM
	To:	DonnaWillis@texashealth.org; Histonet@pathology.swmed.edu
	Subject:	Re: Negative Controls Immunos

	Hi, Donna,  we do not run a negative with each antibody.  It would
run the 
	cost of reagents and tech time up too high.  We do run a positive
with each 
	antibody (our control is on the same slide as the unknown) .  For a
negative, 
	I like to use a sausage various types of tissue... prostate, small
intes, 
	appendix, tonsil, placenta,  etc.    and we use a Rabbit normal
serum or a 
	Mouse normal serum depending on what antibodies are run.  And we
only run one 
	negative.  Or if the antibodies for that case involve rabbit and
mouse, we 
	run one mouse normal serum and one rabbit normal serum.     We just
had a CAP 
	inspection and our inspectors had no problems with the way we did
it.    
	Pearl Gervais