RE: DAB and H&E counterstain

From:Cynthia Favara <>

<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 3.2//EN"> <HTML> <HEAD> <META HTTP-EQUIV="Content-Type" CONTENT="text/html; charset=iso-8859-1"> <META NAME="Generator" CONTENT="MS Exchange Server version 5.5.2653.12"> <TITLE>RE: DAB and H&E counterstain</TITLE> </HEAD> <BODY> <P><FONT SIZE=2>You can add metal. Pierce makes a stable DAB with Nickel. Wonderfully dark. Just one of many options.</FONT> <BR><FONT SIZE=2>Cynthia Favara</FONT> <BR><FONT SIZE=2>Rocky Mountain Laboratories</FONT> <BR><FONT SIZE=2>903 S. 4th Street</FONT> <BR><FONT SIZE=2>Hamilton, MT 59840</FONT> <BR><FONT SIZE=2>406-363-9317</FONT> <BR><FONT SIZE=2>FAX 406-363-9286</FONT> </P> <BR> <P><FONT SIZE=2>-----Original Message-----</FONT> <BR><FONT SIZE=2>From: Michelle Peiffer [<A HREF=""></A>]</FONT> <BR><FONT SIZE=2>Sent: Tuesday, January 30, 2001 11:42 AM</FONT> <BR><FONT SIZE=2>To:</FONT> <BR><FONT SIZE=2>Subject: DAB and H&E counterstain</FONT> </P> <BR> <P><FONT SIZE=2>Hi all,</FONT> </P> <P><FONT SIZE=2>We have a researcher requesting IHC and H&E on the same slide (trying to</FONT> <BR><FONT SIZE=2>save money).  We are using DAB, then trying to counterstain with H&E, and</FONT> <BR><FONT SIZE=2>as expected the DAB is drowned out by the eosin.  Could anybody share a</FONT> <BR><FONT SIZE=2>method of doing this?  Would adding Nickle to the DAB help?  Thanks in</FONT> <BR><FONT SIZE=2>advance for all your good advice.</FONT> </P> <BR> <P><FONT SIZE=2>Michelle Peiffer</FONT> <BR><FONT SIZE=2>*************************************************************</FONT> <BR><FONT SIZE=2>Electron Microscope Facility for the Life Sciences</FONT> <BR><FONT SIZE=2>Penn State University Biotechnology Institute</FONT> <BR><FONT SIZE=2>001 South Frear Lab</FONT> <BR><FONT SIZE=2>University Park PA 16802</FONT> </P> <P><FONT SIZE=2>phone: 814-865-0212</FONT> <BR><FONT SIZE=2>email:</FONT> <BR><FONT SIZE=2>**************************************************************</FONT> <BR><FONT SIZE=2> </FONT> </P> </BODY> </HTML>
<< Previous Message | Next Message >>