RE: DAB and H&E counterstain

From:

Cynthia Favara <cfavara@niaid.nih.gov>

<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 3.2//EN"> <HTML> <HEAD> <META HTTP-EQUIV="Content-Type" CONTENT="text/html; charset=iso-8859-1"> <META NAME="Generator" CONTENT="MS Exchange Server version 5.5.2653.12"> <TITLE>RE: DAB and H&E counterstain</TITLE> </HEAD> <BODY> You can add metal. Pierce makes a stable DAB with Nickel. Wonderfully dark. Just one of many options. Cynthia Favara Rocky Mountain Laboratories 903 S. 4th Street Hamilton, MT 59840 406-363-9317 FAX 406-363-9286 -----Original Message----- From: Michelle Peiffer [<A HREF="mailto:mlk101@psu.edu">mailto:mlk101@psu.edu</A>] Sent: Tuesday, January 30, 2001 11:42 AM To: histonet@pathology.swmed.edu Subject: DAB and H&E counterstain Hi all, We have a researcher requesting IHC and H&E on the same slide (trying to save money). We are using DAB, then trying to counterstain with H&E, and as expected the DAB is drowned out by the eosin. Could anybody share a method of doing this? Would adding Nickle to the DAB help? Thanks in advance for all your good advice. Michelle Peiffer ************************************************************* Electron Microscope Facility for the Life Sciences Penn State University Biotechnology Institute 001 South Frear Lab University Park PA 16802 phone: 814-865-0212 email: mlk101@psu.edu ************************************************************** </BODY> </HTML>

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