Michelle:

Try DAB-Cobalt (we use the Histomark Black Kit), followed by a counterstain
of 

Mayer's Hx for 30 sec.
blue in running tap 3 min.
70% EtOH 6 dips
eosin Y - phloxine B 2 min.
95% EtOH 1 dip
2 changes 100% EtOH 3 min. ea.
xylene
Permount

DAB is black, nuclei dark blue-purple

Hope this helps.
Regards -
Carol
*****************
Carol B. McCollough, HT(ASCP)
Diagnostics & Histology Laboratory Manager
Maryland Department of Natural Resources
Fisheries Service
Cooperative Oxford Laboratory
904 S. Morris Street
Oxford, MD 21654

-----Original Message-----
From: Michelle Peiffer [mailto:mlk101@psu.edu]
Sent: Tuesday, January 30, 2001 1:42 PM
To: histonet@pathology.swmed.edu
Subject: DAB and H&E counterstain


Hi all,

We have a researcher requesting IHC and H&E on the same slide (trying to
save money).  We are using DAB, then trying to counterstain with H&E, and
as expected the DAB is drowned out by the eosin.  Could anybody share a
method of doing this?  Would adding Nickle to the DAB help?  Thanks in
advance for all your good advice.


Michelle Peiffer
*************************************************************
Electron Microscope Facility for the Life Sciences
Penn State University Biotechnology Institute
001 South Frear Lab
University Park PA 16802

phone: 814-865-0212
email:  mlk101@psu.edu
**************************************************************
 
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