DAB and H&E counterstain

From:Michelle Peiffer <mlk101@psu.edu>

Hi all,

We have a researcher requesting IHC and H&E on the same slide (trying to
save money).  We are using DAB, then trying to counterstain with H&E, and
as expected the DAB is drowned out by the eosin.  Could anybody share a
method of doing this?  Would adding Nickle to the DAB help?  Thanks in
advance for all your good advice.

Michelle Peiffer
Electron Microscope Facility for the Life Sciences
Penn State University Biotechnology Institute
001 South Frear Lab
University Park PA 16802

phone: 814-865-0212
email:  mlk101@psu.edu

<< Previous Message | Next Message >>