Hi all,

We have a researcher requesting IHC and H&E on the same slide (trying to
save money).  We are using DAB, then trying to counterstain with H&E, and
as expected the DAB is drowned out by the eosin.  Could anybody share a
method of doing this?  Would adding Nickle to the DAB help?  Thanks in
advance for all your good advice.


Michelle Peiffer
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Electron Microscope Facility for the Life Sciences
Penn State University Biotechnology Institute
001 South Frear Lab
University Park PA 16802

phone: 814-865-0212
email:  mlk101@psu.edu
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