I don't know what species Louri is working on, but poly-CD3 works well for
me on dog, cat, pig, cow, horse, monkey, baboon.

Jan

----- Original Message -----
From: "Gayle Callis" <uvsgc@montana.edu>
To: "Jan Shivers" <shive003@maroon.tc.umn.edu>;
<histonet@pathology.swmed.edu>
Sent: Tuesday, January 30, 2001 10:36 AM
Subject: Re: CD3 for Veterinary Medicine


> Maybe I missed it, but what species are you working with, dog, cat, horse,
> bovine, etc???? for this CD3 staining?
>
>
>
> At 09:18 AM 1/30/01 -0600, you wrote:
> >Louri,
> >
> >I use the polyclonal CD3 from DAKO; microwave pretreatment in citrate
> >buffer - 2 x 5 minutes with 20 minute cooldown; H2O2 block for 15
minutes;
> >normal goat serum protein block for 15 minutes prior to primary antibody;
> >CD3 at 1:50 for 45 minutes, LSAB2 link and label for 15 minutes each; AEC
> >for 10 minutes.  All steps from H2O2 block on - done on autostainer.  I
make
> >my CD3 dilution from concentrate right before each time I use it.
> >
> >Jan Shivers
> >U of MN Vet Diag Lab
> >  ----- Original Message -----
> >  From: Louri Caldwell
> >  To: histonet@pathology.swmed.edu
> >  Sent: Monday, January 29, 2001 7:52 PM
> >  Subject: CD3 for Veterinary Medicine
> >
> >
> >  Hello everyone,
> >
> >  We currently are having inconsistencies with our CD3 marker.  I was
> >wondering if  any of you may be using an antibody that is providing good
> >results?  I would like to try another source, and any suggestions you may
> >have would be greatly appreciated.
> >
> >  Thank you in advance for your help.
> >
> >  Louri Caldwell
> >
> >
>
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> Gayle Callis
> Veterinary Molecular Biology
> Montana State University - Bozeman
> Bozeman MT 59717-3610
>
> 406 994-6367
> 404 994-4303 (FAX)
>
>