eosinophil staining on frozen sections

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From:CKlemenich@chob.edu (by way of Marvin Hanna)
To:histonet@histosearch.com
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Does anyone have a method  for  intensifing the staining of the eosinophils
on a frozen section  using the standard  H & E ?  Would adjusting the pH of
the eosin help stain the eosinophils better ?  The pathologist here have
been asking this question for years and I do not have an answer.

Chris Klemenich
Kaleida Health - Childrens Hospital of Buffalo
ckemenich@kaleidahealth.org




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