Re: eosinophil staining on frozen sections
<< Previous Message | Next Message >>
From: | "Jamie Erickson" <JErickson@genetics.com> (by way of Marvin Hanna) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
I am trying to stain mouse eosinophil in formalin fixed paraffin embedded
tissues. I am using a dye called Vital new red from an Article in the
Journal of Histotechnology by J.P.Duffy, Vol 16/ No. 2/June 1993. The
article stains human Eosinophil a nice dark red which I'm tring on my mouse
tissues and getting inconsistent results. After talking to other people
including ( Gayle Callis) about differences in human eosinophil and mouse
eosinophil , I'm thinking that this dye vital new red may have to be
adjusted to stain mouse eos, that's what I'm doing now. This dye has not
stained a dark red on positive control tissues it has been a weak pink at
best. If anyone has experience with this stain or can offer suggestions
please e-mail me.
Thanks
Jamie Erickson
Associate Scientist
Genetics Institute
1 Burtt Rd
Andover, MA 01810
(978) 247-1348
FAX (978) 247-1333
>>> <CKlemenich@chob.edu> 01/24 10:29 AM >>>
Does anyone have a method for intensifing the staining of the eosinophils
on a frozen section using the standard H & E ? Would adjusting the pH of
the eosin help stain the eosinophils better ? The pathologist here have
been asking this question for years and I do not have an answer.
Chris Klemenich
Kaleida Health - Childrens Hospital of Buffalo
ckemenich@kaleidahealth.org
<< Previous Message | Next Message >>