Re: Quick Question!

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From:Dana Settembre <settembr@UMDNJ.EDU> (by way of Marvin Hanna)
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On Thu, 27 Jan 2000 wrote:

>      Hi Histonetters!  I just have a quick question, mostly prompted by
>my own
> personal curiosity!  When performing immunhistochemical experiments by
>hand (I
> know, a bit antiquated but I have no choice) what kind of staining racks are
> people using? Thanks as always for all the generous and kind input!
> Jennifer Hoover
> Novartis Pharmaceuticals
Hi Jennifer
I used to run immunos by hand and very rarely, but sometimes, still do. In
reference to staining racks, I have used metal or glass staining racks for
most of the steps, such as deparaffinizing, quenching,
counterstaining, and dehydrating.   I have used "rinsing trays" or
Shandons Sequenza "racks" for applying antibodies.

Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey

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