Re: Quick Question!
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From: | Dana Settembre <settembr@UMDNJ.EDU> (by way of Marvin Hanna) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
On Thu, 27 Jan 2000 jennifer.hoover@pharma.Novartis.com wrote:
> Hi Histonetters! I just have a quick question, mostly prompted by
>my own
> personal curiosity! When performing immunhistochemical experiments by
>hand (I
> know, a bit antiquated but I have no choice) what kind of staining racks are
> people using? Thanks as always for all the generous and kind input!
>
>
> Jennifer Hoover
> Novartis Pharmaceuticals
>
>
>
Hi Jennifer
I used to run immunos by hand and very rarely, but sometimes, still do. In
reference to staining racks, I have used metal or glass staining racks for
most of the steps, such as deparaffinizing, quenching,
counterstaining, and dehydrating. I have used "rinsing trays" or
Shandons Sequenza "racks" for applying antibodies.
Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark, New Jersey
USA
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