H. pylori stain (Alcian yellow; Why?)

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From:"J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of Marvin Hanna)
To:histonet@histosearch.com
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On Fri, 14 Jan 2000, Amos Brooks wrote a full set of instructions,
from which a few selected lines are now quoted:

> In order to not be information stingy I'm posting the Alcian Yellow
> procedure to everyone. :-)

> RESULTS
>     Helicobacter pylori --- Blue
_______________________________

  Amos's description of the technique will be very useful
  for those without access to journals or libraries.

  BUT (and I don't often shout) why is this called the
  alcian yellow method when the bacteria are stained by
  toluidine blue?  This seems equivalent to the "eosin
  method for nuclei," or the "alum-hematoxylin method for
  cytoplasm and collagen."
                             John Kiernan
                             London, Canada











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