H. pylori stain (Alcian yellow; Why?)
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of Marvin Hanna) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
On Fri, 14 Jan 2000, Amos Brooks wrote a full set of instructions,
from which a few selected lines are now quoted:
> In order to not be information stingy I'm posting the Alcian Yellow
> procedure to everyone. :-)
> RESULTS
> Helicobacter pylori --- Blue
_______________________________
Amos's description of the technique will be very useful
for those without access to journals or libraries.
BUT (and I don't often shout) why is this called the
alcian yellow method when the bacteria are stained by
toluidine blue? This seems equivalent to the "eosin
method for nuclei," or the "alum-hematoxylin method for
cytoplasm and collagen."
John Kiernan
London, Canada
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