Andi,

Are you washing the slides in running water after they have been in nuclear 
fast red? I have found that if you don't wash sufficiently, there will be 
white precipitate on the slides.




At 08:43 AM 1/5/2001 -0700, Andrea Grantham wrote:
>Histonetters -
>I was wondering if there was another stain (other than Nuclear Fast Red) 
>that could be used as a counterstain for the iron stains?
>My next question is - what can be done to prevent the white precipitate 
>from adhering to the slides that have been in Nuclear Fast Red 
>(Kernechtrot)Solution? I have had this problem with several batches of the 
>stain - not just in this lab with these chemicals. I am sure that I am 
>making up the solution correctly and just to be certain I had another tech 
>make it up using the "recipe" from the green AFIP book (page 179). Still 
>the same problem.
>Any advice/help would be greatly appreciated since this is driving me nuts!
>Thanks!
>Andi
>.....................................................................
>: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
>: Sr. Research Specialist       University of Arizona               :
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>


**************************************************************************
Jill Songer HT (ASCP)
Virginia Tech
Veterinary Teaching Hospital
Anatomic Pathology Lab Supervisor
Blacksburg, Virginia 24061

GO HOKIES!!!!!!