If you rinse well with DI water you should not get the precipitate.

Karla Carson
Regional Pathology Manager
Mercy Health Care Sacramento
Phone 916-453-4494
FAX 916-453-4397
e-mail kcarson@chw.edu <mailto:kcarson@chw.edu> 

		-----Original Message-----
		From:	Andrea Grantham [mailto:algranth@u.arizona.edu]
		Sent:	Friday, January 05, 2001 7:44 AM
		To:	histonet@pathology.swmed.edu
		Subject:	Iron stain counterstain

		Histonetters -
		I was wondering if there was another stain (other than
Nuclear Fast Red) 
		that could be used as a counterstain for the iron stains?
		My next question is - what can be done to prevent the white
precipitate 
		from adhering to the slides that have been in Nuclear Fast
Red 
		(Kernechtrot)Solution? I have had this problem with several
batches of the 
		stain - not just in this lab with these chemicals. I am sure
that I am 
		making up the solution correctly and just to be certain I
had another tech 
		make it up using the "recipe" from the green AFIP book (page
179). Still 
		the same problem.
		Any advice/help would be greatly appreciated since this is
driving me nuts!
		Thanks!
		Andi
	
.....................................................................
		: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology &
Anatomy     :
		: Sr. Research Specialist       University of Arizona
:
		: (office:  AHSC 4212)          P.O. Box 245044
:
		: (voice:  520-626-4415)        Tucson, AZ  85724-5044
USA       :
		: (FAX:  520-626-2097)          (email:
algranth@u.arizona.edu)       :
	
:...................................................................:
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