Hi Martha,

We use Zymed's V-Line predilute antibody (cat. # 08-4211) on Ventana's ES
and genII instruments with Ventana detection (of course!)  Our HIER
procedure is 2" in 1mM EDTA, pH 8 in the Biocare Decloaking chamber (you
could substitute a 93-95 degree C waterbath for 20-30" if you don't have an
electric pressure cooker). We incubate the primary Ab for a 20" titration
and add AB block to the procedure.  Mesothelioma cases come out very
intense, yet clean.

Feel free to contact us if you have further questions.

Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, D4/218
600 Highland Ave.
Madison, WI 53792-2472
(608)265-6596
FAX: (608)263-1568
<la.sebree@hosp.wisc.edu>


-----Original Message-----
From: mward@wfubmc.edu [mailto:mward@wfubmc.edu]
Sent: Wednesday, January 03, 2001 8:53 AM
To: histonet@pathology.swmed.edu
Subject: calretinin


Martha Ward writes:  I am looking for a source for a calretinin antibody
that would work well on our Ventana ES.  We currently use the one from
Zymed, along with their detection system.  This works well but with our
increasing workload I need something we can automate.  Any help would be
greatly appreciated.