Direct red 72 and Pagoda red
|From:||ANATECH LTD <firstname.lastname@example.org>|
After several years of research involving both empirical staining and
theoretical deductions from molecular modeling, we have found direct
red 72 to be superior to Congo red and Sirius red F3B for staining
amyloid. This dye produces a better color than Congo red, offering
improved contrast. It is birefringent and fluorescent, although we
have not really felt the need to go to these specialized optical
methods to visualize tiny deposits. Both Congo red and Sirius red
F3B present solubility problems which are much reduced with direct
red 72. Finally, we have a highly specific staining protocol that is
simpler than most Congo red methods.
Anatech Ltd. plans to offer this dye for sale in the United States
sometime in the next few months. Directions for solution preparation
and staining will be included. Interested parties may contact us
directly. To my knowledge, this dye is not available from another
source in laboratory quantities.
Pagoda red, mentioned by Terry Marshall, is (was?) a Bristish dye
manufactured by Dylon for home textile dyeing. Its composition is
not revealed, but like similar Rit dyes in the USA, it most probably
is a mixture of acid and direct dyes whose exact composition varied
from lot to lot to meet changing market demands, if in fact more than
one batch existed. I had a 1 gram sample dating back to the 1960's.
Scattered references in the histological literature may be found, all
without concrete information. Apparently someone tried it, it
worked, and passing mention was made; thus Pagoda red entered the
Richard W. Dapson, Ph.D.
Battle Creek, MI
Web address: anatechltdusa.com
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