Here's our vote-

For NBF-fixed paraffin blocks 1 mM EDTA HIER in waterbath or pressure
cooker.
For B5-fixed decal'd bone marrow biopsies first we use BioGenex's DeCal
Retrieval Solution, then HIER in 1mM EDTA in a pressure cooker...We use a
monoclonal CD3(1:10) from BioGenex and our detection is on the Ventana
ES/genII... We use their amplification kit also...

Sally Ann Drew-MT(ASCP)
Univ. of Wis. Hospital and Clinics
IHC/ISH Clinical/Research Lab
Madison, WI
(608)265-6596
(608)263-1568(fax)

> -----Original Message-----
> From:	Richard Cartun [SMTP:Rcartun@harthosp.org]
> Sent:	Thursday, January 06, 2000 9:35 AM
> To:	Histonet@pathology.swmed.edu
> Subject:	CD3
>
> I am interested in hearing what Histoneters are using for the detection of
> CD3+ cells in paraffin sections (pAb vs. mAb, digestion vs. HIER, etc.).
> Thanks.
>
> R. Cartun