After making this up with the expense of chemicals, plus storing them, it
was much easier to buy it in bulk from Poly Scientific, a histo reagent
supplier, don't have the 800 number offhand, but it was reasonable for
price and quantity.  The buffer is sold separate from fixative, need both
which you are probably already aware of. 

  


At 02:28 PM 1/12/01 -0600, you wrote:
>This is what I have:
>
>Michels Buffer
>1M potassium citrate, pH 7.0            2.5 ml
>0.1M magnesium sulfate                   5.0 ml
>0.1M N-ethyl malemide                      5.0 ml
>d H2O                                                 87.5 ml
>* Mix well and store at 2-8C.   Exp. 1 year
>
>Michel's Fixative
>Michel's Buffer                                    100ml
>ammonium sulfate                                55gm
>Adjust pH to 7.0-7.4 with 1M KOH.  Store at 2-8C.  Exp. 1 year
>
>Bonnie Whitaker
>UT--Houston
>
>----- Original Message -----
>From: Atoska Gentry <gentras@vetmed.auburn.edu>
>To: <histonet@pathology.swmed.edu>
>Sent: Friday, January 12, 2001 11:50 AM
>Subject: michele's fixative
>
>
>> Please will someone  provide me with a recipe for michele's fixative used
>> for immunoflourescence ASAP?  Thanks, Atoska
>>
>> Atoska  S. Gentry B.S., HT(ASCP)
>> Research Assistant II
>> Scott-Ritchey RSCH Center
>> Auburn University, AL  36849
>> PH:   (334) 844-5579
>> Fax:  (334) 844-5850
>>
>>
>
>
>
>
Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303