I agree with Gail.  You can purchase from NewComer Supply as well,
1-800-383-7799

Diane
millerd@coho.net


----- Original Message -----
From: "Gayle Callis" <uvsgc@msu.oscs.montana.edu>
To: "Bonnie P Whitaker" <Bonnie.P.Whitaker@uth.tmc.edu>;
<histonet@pathology.swmed.edu>
Sent: Friday, January 12, 2001 8:01 AM
Subject: Michel's fixative


> After making this up with the expense of chemicals, plus storing them, it
> was much easier to buy it in bulk from Poly Scientific, a histo reagent
> supplier, don't have the 800 number offhand, but it was reasonable for
> price and quantity.  The buffer is sold separate from fixative, need both
> which you are probably already aware of.
>
>
>
>
> At 02:28 PM 1/12/01 -0600, you wrote:
> >This is what I have:
> >
> >Michels Buffer
> >1M potassium citrate, pH 7.0            2.5 ml
> >0.1M magnesium sulfate                   5.0 ml
> >0.1M N-ethyl malemide                      5.0 ml
> >d H2O                                                 87.5 ml
> >* Mix well and store at 2-8C.   Exp. 1 year
> >
> >Michel's Fixative
> >Michel's Buffer                                    100ml
> >ammonium sulfate                                55gm
> >Adjust pH to 7.0-7.4 with 1M KOH.  Store at 2-8C.  Exp. 1 year
> >
> >Bonnie Whitaker
> >UT--Houston
> >
> >----- Original Message -----
> >From: Atoska Gentry <gentras@vetmed.auburn.edu>
> >To: <histonet@pathology.swmed.edu>
> >Sent: Friday, January 12, 2001 11:50 AM
> >Subject: michele's fixative
> >
> >
> >> Please will someone  provide me with a recipe for michele's fixative
used
> >> for immunoflourescence ASAP?  Thanks, Atoska
> >>
> >> Atoska  S. Gentry B.S., HT(ASCP)
> >> Research Assistant II
> >> Scott-Ritchey RSCH Center
> >> Auburn University, AL  36849
> >> PH:   (334) 844-5579
> >> Fax:  (334) 844-5850
> >>
> >>
> >
> >
> >
> >
> Gayle Callis
> Veterinary Molecular Biology
> Montana State University
> Bozeman MT 59717-3610
> 406 994-4705
> 406 994-4303
>