-----Original Message-----
From: HistoNet Server <HistoNet@Pathology.swmed.edu>
To: HistoNet Server <HistoNet@pathology.swmed.edu>
Date: Monday, February 15, 1999 6:32 AM
Subject: Daily Digest


>
>----------------------------------------------------------------------
>
>Date: 14 Feb 1999 04:00:20 -0600
>From: DORIT ZHARHARY <d_zharhary@sigma.co.il>
>Subject: RE: Question
>
>Lynn,
>
>Sigma has 2 new monoclonal antibodies to the KDR - VEGF-receptor 2. You can
>find them in the immunochemical section of the Sigma catalog.
> Product number V3003, clone KDR-2 - works well in immuno-histo and
western.
> Product number V9134 clone KDR-1 - works well by western blotting.
>
>Good Luck,
>
>Dorit
>- ----------
>From:  Lynn Gardner
>Sent:  eai cieue 11 oaoaao 1999 18:40
>To:  histonet@pathology.swmed.edu
>Subject:  Question
>
>Hey, Guys and Gals, I have a question concerning the antibody KDR (Kinase
>Domain containing Receptor) can anyone give me the name of a company which
>would carry this product. I have already looked in the following
>catalogues: Vector, Novacastra, Dako, Biogenex, Biogenisis, BioMeda,
>Jackson, Oncogene, Pharmagen, Sero Tec, R&D Systems and Zymed. I have also
>looked in the Linn Scott directory and have found nothing. Thanks for your
>help!!
>
>Lynn Gardner
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 14 Feb 1999 10:00:46 -0600
>From: "Rae Staskiewicz" <raestask@galesburg.net>
>Subject: 1999 Illinois Society for Histotechnologists Meeting
>
>I would like to announce the 1999 Illinois Society for Histotechnologists
>annual meeting, to be held May 13-15,1999. The site will be Jumer's Castle
>Lodge, Peoria, IL. Some of the presenters this year will be Dr. Frieda
>Carson, Ada Feldman, Gwen Goss, Jan Minshew, and others. We will also enjoy
>an evening of chance aboard the Par-A-Dice riverboat casino. If you would
>like to try your luck, send your mailing address and a program will be sent
>to you. We will be mailing the programs the first part of March. Hope to
see
>you there!
>
>Rae Ann Staskiewicz
>President
>Illinois Society for Histotechnologists
>
>
>
>----------------------------------------------------------------------
>
>Date: 14 Feb 1999 10:30:49 -0600
>From: elephantsneedus@erols.com
>Subject: Sirius Red method for amyloid
>
>Does anyone have a shortened protocol for Sirius Red for amyloid.
>
>Thanks!
>
>Marian
>
>
>----------------------------------------------------------------------
>
>Date: 14 Feb 1999 11:45:29 -0600
>From: krenek@gtwn.net
>Subject: disposal
>
>I would like to know if other histo departments have a disposal in the
>sink at the grossing station.  Every other hospital I have ever workd at
>has, but at my current job we  have been told that it is an infectious
>hazard and we could not get one.  It seems to me having to pick the all
>the lovely stuff out of the sink drain by hand is more of a hazard.  Is
>this something new I don't know about.  I would like to have some facts
>to take to my supervisor on this.  Thanks for the help.
>
>Pam Krenek
>
>
>
>----------------------------------------------------------------------
>
>Date: 14 Feb 1999 12:00:33 -0600
>From: "Bryan Llewellyn" <bryand@netbistro.com>
>Subject: Re: Sirius Red method for amyloid
>
>
>- -----Original Message-----
>From: gp <elephantsneedus@erols.com>
>To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
>Date: February 14, 1999 8:34 AM
>Subject: Sirius Red method for amyloid
>
>
>>Does anyone have a shortened protocol for Sirius Red for amyloid.
>>
>>Thanks!
>>
>>Marian
>>
>>
>
>Sirius red can be used in a Highman's congo red type procedure quite
>effectively, as can many other of the "sirius" direct cotton dyes.  Total
>time to stain is about 15 minutes.
>
>Solutions
>0.5% Congo red (or Sirius red) in 50% ethanol
>0.2% potassium hydroxide in 70% ethanol
>Mayers alum hematoxylin or another progressive type.
>
>
>Method
>1.    Sections to water.
>2.    Stain nuclei for 2 minutes, no need to blue.
>3.    Place in dye solution for 5 minutes or longer.
>4.    Rinse with water.
>5.    Place into alkaline ethanol until dye ceases to extract - a few
>minutes.
>6.    Rinse well with water.
>7.    Dehydrate, clear and mount.
>
>Amyloid - red
>Nuclei - blue
>
>
>Bryan Llewellyn
>
>
>
>----------------------------------------------------------------------
>
>Date: 14 Feb 1999 15:15:44 -0600
>From: BCPCOMMUN@aol.com
>Subject: AUTOMATIC MICROTOMES
>
>My lab is currently beginning to demo automatic microtomes.  I will
appreciate
>information on ones you may now be using and your experience with service,
>support and reliability of various brands.
>
>Thanks,
>Carol Panken
>bcpcommun@aol.com
>
>
>----------------------------------------------------------------------
>
>Date: 14 Feb 1999 16:15:50 -0600
>From: Nadania@aol.com
>Subject: Re: credit hrs. vs. contact hours
>
>Here's an easy question for all you who are involved in the education
aspect
>of histology: how do "contact hours" and credit hours compare to each
other?
>Does one credit hour equal one contact hour? Thank you in advance!
>
>
>----------------------------------------------------------------------
>
>Date: 14 Feb 1999 17:15:48 -0600
>From: "Mick Rentsch" <ausbio@nex.com.au>
>Subject: Re: crowded laboratory (mice)
>
>When the Overland telegraph was first set up in Australia and for the next
>twenty years, all telephonists were Male, in the 1950's & 1960's they were
>all female, today in the nineties the mix is about 60/40 m/f.
>The main difference is I believe, is that ladies are more facile and are
>more fastidious about detail which is afterall something I seek in
>prospective applicants for Histo.
>Regards Mike (downunder)
>- -----Original Message-----
>From: DayDawning@aol.com <DayDawning@aol.com>
>To: jkiernan@julian.uwo.ca <jkiernan@julian.uwo.ca>; cbuddyh@mindspring.com
><cbuddyh@mindspring.com>
>Cc: histonet@Pathology.swmed.edu <histonet@Pathology.swmed.edu>
>Date: Friday, 12 February 1999 12:17
>Subject: Re: crowded laboratory (mice)
>
>
>>In a message dated 2/11/99 2:33:25 AM Eastern Standard Time,
>>jkiernan@julian.uwo.ca writes:
>>
>><<  The womice came in later, when they were
>>   the majority, and gnawed off the testes of all but the fastest
>>   gentlemice. Thus, genes for aggression and speed got passed
>>   on to the mildren, and this is still going on ....
>>  >>
>>Would this theory explain why the majority of the folks in the field of
>>histology are women?
>>Dawn
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 14 Feb 1999 17:16:15 -0600
>From: "Mick Rentsch" <ausbio@nex.com.au>
>Subject: Re: crowded laboratory
>
>Dear Terence,
>although I am retired from the Histo-lab, I can tell you that I have worked
>both in a very crowded Lab and in a very spacious one, with approx the same
>numbers in both. One thing I learnt very early it is the function of the
lab
>as a team that really counts and that in the cramped original lab we came
to
>know each others habits etc. so well that we were always very tolerant of
>each other; when we had to break in a new person not all, so that those
that
>didn't get on were found a job elsewhere, I know this sounds very "Clicky"
>but it was essential and we could not afford a clash of wills etc. in such
a
>small area and I dislike having to bully people into pulling their own
>weight.
>when we moved into a much larger lab, we carried on in much the same
>fashion, except that management decided to rotate Lab. Assisants through
our
>section on a 12 week basis, most of the time this only caused extra time
>teaching someone the donkey work, but there were times when we could'nt
wait
>for the end of that particular twelve week cycle to come to an end.
>After twenty years of harmonious working together, a peer review system of
>staff appraisal was introduced through the entire practice, and with it cam
>e such a degree of division and mistrust that both efficency and quality
>have suffered and the prime focus the patient has been placed to the side
in
>favour of personal interests, position, advancement etc.
>Although retiring was one of the hardest things I've had to do, I'm glad I
>did it.
>In summary, I would like to suggest to you that you also look closely at
>factors such appraisal systems, peer reviews etc. and examine their impact
>and changes etc. c/w Labs that do not etc. But please remember the Patient
>should always be the prime focus. And a final observation "Ants and bees
>live and work in a cramped envioronment and still manage to work as an
>efficient team" so while a mice model may offer some insight, there are
>other models.
>Regards Mike (Downunder)
>- -----Original Message-----
>From: Terence Murphy <cbuddyh@mindspring.com>
>To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
>Date: Thursday, 11 February 1999 2:18
>Subject: crowded laboratory
>
>
>>Hello histologists
>>
>>
>>IM working on my masters thesis through St. Joes. University in
>>Philladelphia PA.  My thesis topic is the effect of a crowded work place
on
>>morale and productivity.  I have compiled a surveya and I'm looking for a
>>crowded hospital laboratory to participate in my project.  If you are
>>interested please contact me.
>>
>>Thank You
>>
>>Terry Murphy
>>email: cbuddyh@mindspring.com
>>work # 610-378-6565
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 14 Feb 1999 17:16:42 -0600
>From: "Mick Rentsch" <ausbio@nex.com.au>
>Subject: Re: Demonstration of amylase
>
>Dear Jim,
>I know I sound niave, but what would happen if you applied schiff reagent
to
>the section (No Oxidation Step), gave it a good rinse in 0.5% Sodium
>Metabisulphite (Fresh) and then examined it. Wouldn't work would it,
because
>no sugar, mucin or glycogen necessarily at site, and if was incorporated in
>solution before application of schiff would have to bind to site  and be
>insoluble.
>Could you use an anti-amylase generated antibody  if there is such a thing?
>with a 1-5 glycosidic linkage sugar attached, then the glycogen like
>material would be bound to the site and visualise with the schiff reagent.
>My suggestion is probably pie in the sky stuff, but who knows?
>Regards Mike (Downunder)
>- -----Original Message-----
>From: Jim Almond <jaa@rshhis.demon.co.uk>
>To: histonet@pathology.swmed.edu <histonet@pathology.swmed.edu>
>Date: Thursday, 11 February 1999 4:50
>Subject: Demonstration of amylase
>
>
>>Hi everyone
>>
>>We have been asked to demonstrate sites of amylase activity on frozen
>>tissue sections. Help would be appreciated if anyone has a reliable
method.
>>An initial trial of starch film methods is causing problems with
>>reproducibility. Any tips, advice or alternative approaches gratefully
>>received.
>>
>>Jim Almond
>>Royal Shrewsbury Hospital
>>UK
>>Jim Almond
>>Tel: +44 (0) 1743 261168  Fax: +44 (0) 1743 355963
>>Email: jaa@rshhis.demon.co.uk
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 14 Feb 1999 18:45:56 -0600
>From: William scarrow <wigo@Atcon.com>
>Subject:
>
>can anyone refer me to articles on the ihc on cytology specimens & blood
>smears on bone marrow aspirates, or anyone routinely performing ihc on
>theses types of specimens, please contact me through histonet.
>
>
>
>----------------------------------------------------------------------
>
>Date: 14 Feb 1999 20:16:07 -0600
>From: "Bob Bacon" <BBacon@prodigy.net>
>Subject: Open position
>
>Hello all:
>We have a research histologic technician position open at the University of
>Missouri in Columbia Missouri.  This position is responsible for embedding,
>cutting, and staining research tissue for the Research and Diagnostic
>Investigative Laboratory here at the University located in the School of
>Veterinary Medicine.  We also prepare and stain slides for independent
>investigators both from the University of Missouri as well as research
>tissue from other Universities and private corporations doing research.
>
>We are a small laboratory that is completely automated.  We use Micron
>automated microtomes.  If you've ever battle repetitive motion problems,
>these microtomes are an answer to your prayers.  We have an automated
>coverslipper and stainer.  We're very progressive in having a safe, well
>ventilated laboratory with excellent working conditions, good pay and
>excellent benefits.  Because we do research versus clinical, we have a
>flexible work schedule and work Monday through Friday with no weekend work.
>
>To download the posting via the internet go to
>http://www.missouri.edu/~hrsww/50441.htm
>
>This will list all pertinent qualifications and salary ranges as well as
>having a section to download an application form.
>
>Sincerely yours,
>Bob Bacon HT ASCP
>573-882-6438
>University of Missouri
>
>
>
>----------------------------------------------------------------------
>
>Date: 14 Feb 1999 20:25:52 -0600
>From: "Bob Bacon" <BBacon@prodigy.net>
>Subject: Fw: Open position
>
>
>Date: Sunday, February 14, 1999 7:59 PM
>Subject: Open position
>
>
>>Hello all:
>>We have a research histologic technician position open at the University
of
>>Missouri in Columbia Missouri.  This position is responsible for
embedding,
>>cutting, and staining research tissue for the Research and Diagnostic
>>Investigative Laboratory here at the University located in the School of
>>Veterinary Medicine.  We also prepare and stain slides for independent
>>investigators both from the University of Missouri as well as research
>>tissue from other Universities and private corporations doing research.
>>
>>We are a small laboratory that is completely automated.  We use Micron
>>automated microtomes.  If you've ever battle repetitive motion problems,
>>these microtomes are an answer to your prayers.  We have an automated
>>coverslipper and stainer.  We're very progressive in having a safe, well
>>ventilated laboratory with excellent working conditions, good pay and
>>excellent benefits.  Because we do research versus clinical, we have a
>>flexible work schedule and work Monday through Friday with no weekend
work.
>>
>>To download the posting via the internet go to
>>http://www.missouri.edu/~hrsww/50441.htm
>>
>>This will list all pertinent qualifications and salary ranges as well as
>>having a section to download an application form.
>>
>>Sincerely yours,
>>Bob Bacon HT ASCP
>>573-882-6438
>>University of Missouri
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 14 Feb 1999 20:26:21 -0600
>From: "Bob Bacon" <BBacon@prodigy.net>
>Subject: Fw: Open position
>
>
>Date: Sunday, February 14, 1999 7:59 PM
>Subject: Open position
>
>
>>Hello all:
>>We have a research histologic technician position open at the University
of
>>Missouri in Columbia Missouri.  This position is responsible for
embedding,
>>cutting, and staining research tissue for the Research and Diagnostic
>>Investigative Laboratory here at the University located in the School of
>>Veterinary Medicine.  We also prepare and stain slides for independent
>>investigators both from the University of Missouri as well as research
>>tissue from other Universities and private corporations doing research.
>>
>>We are a small laboratory that is completely automated.  We use Micron
>>automated microtomes.  If you've ever battle repetitive motion problems,
>>these microtomes are an answer to your prayers.  We have an automated
>>coverslipper and stainer.  We're very progressive in having a safe, well
>>ventilated laboratory with excellent working conditions, good pay and
>>excellent benefits.  Because we do research versus clinical, we have a
>>flexible work schedule and work Monday through Friday with no weekend
work.
>>
>>To download the posting via the internet go to
>>http://www.missouri.edu/~hrsww/50441.htm
>>
>>This will list all pertinent qualifications and salary ranges as well as
>>having a section to download an application form.
>>
>>Sincerely yours,
>>Bob Bacon HT ASCP
>>573-882-6438
>>University of Missouri
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 14 Feb 1999 20:47:02 -0600
>From: "Bob Bacon" <BBacon@prodigy.net>
>Subject: Micron microtome
>
>Dear Rae:
>Two things that I can think of.
>1.  You have the blade holder cranked down too tight.  It just needs to be
>snug.  Not really tight.
>2nd.  The angle of our knife holder that we favor is 11 degrees.  That
seems
>to work the best for us.  I'll ask the other techs for ideas tomorrow at
>work.  Not only do we use Micron microtomes in our Research Lab.  They also
>use them over in the diagnostic lab as well.  Hope this helps.
>Take care.  Sincerely yours, Bob
>- -----Original Message-----
>From: Rae Staskiewicz <raestask@galesburg.net>
>To: Bob Bacon <BBacon@prodigy.net>
>Date: Sunday, February 14, 1999 8:22 PM
>Subject: Re: Open position
>
>
>>Dear Bob,
>>
>>    I see by your posting that you have Microm automatic microtomes.
Having
>>recently received just such a machine, I have a question perhaps you can
>>answer. I seem to be getting a lot of venetian blind artifact. I have
>>tightened the blade holder as tight as I can get it. I have tried four
(4!)
>>different makes of blades, and nothing seems to work. The company has
>loaned
>>me a new blade holder and still I get the artifact. EXCEPT, when I operate
>>it manually. Can you or any of your techs, think of what I can be doing
>>wrong. This is just about to drive me crazy!!!
>>
>>
>>Thanks in advance,
>>Rae Ann Staskiewicz
>>Galesburg Animal Disease Lab
>>Galesburg, IL
>>
>
>
>
>----------------------------------------------------------------------
>
>Date: 15 Feb 1999 08:00:06 -0600
>From: "bbracing" <bbracing@silk.net>
>Subject: Rae Staskiewcz - Re microm Microtome
>
>Rae -  I have two microm automatics, and just love them.  The problem that
you
>are having may be the knife holder.  I have found that these holders are
very
>easily damaged, and even the smallest imperfection in the clamping surface
>will cause a vibration problem.  This is because, an imperfection will
cause
>the blade to be clamped tightly in one spot, but not the rest of the blade,
so
>it permits the blade to vibrate.  I have a small machinest dressing file,
>about 5 inches long that I use to dress the imperfections out of both the
>lower clamping surface and the upper clamping surface. Quite often, when
you
>run the file across these surfaces you will immediately see that it is
>removing very small amounts of metal in only one spot. Dress the holder
down
>untll the entire surface shows new dressing marks.
>There is a second problem that I had with one of my units that is a little
>more difficult to detect.  With this unit the upper clamping plate  griped
the
>blade about 1/2 way down, instead of at the upper most portion.  Again this
>let the blade vibrate when cutting any thing but the softest of tissues.
To
>determin if this is the problem, you can try to observe the clamping plate
and
>knife end on, to see if the clamping plate is in fact clamping the blade at
>its upper most edge.  Or, you can use some very thin feeler gauges ( 0.01 -
>0.02 ) to see if you have a clearance problem, or in my case I had to
revert
>to some machinests blueing which I painted on the underside of the upper
>clamping plate, then clamped a blade in the holder. The blueing transfers
onto
>the blade, and shows you exactly where the pressure points of the clamp
are.
>If this is the problem, then you have to have the clamping plate
reconfigured,
>( I simply filed mine ) untill it clamps the blade properly, which is along
>the upper most portion of the the clamping plate as close to the cutting
edge
>of the blade as possible.  On more thing, with these units (and using
feather
>blades) I have found that they seam to cut best when the holder is showing
13
>degrees of  knife angle.
>Hope this is of some help.
>Kerry Beebe
>Kelowna Gen Hospital
>Kelowna B.C. Canada
>bbracing@silk.net
>
>
>
>----------------------------------------------------------------------
>
>Date: 15 Feb 1999 08:01:13 -0600
>From: Ian Montgomery <ian.montgomery@bio.gla.ac.uk>
>Subject: gluteraldehyde - Paraffin?
>
>>Date: Fri, 12 Feb 1999 12:34:04 -0600
>>From: Mary Vaughan <mvaughan@sc3102.med.buffalo.edu>
>>Subject: gluteraldehyde - Paraffin?
>>To: HistoNet@pathology.swmed.edu
>>MIME-version: 1.0
>>
>>Someone just inquired: If a tissue is in gluteraldehyde, can it be
processed
>>for paraffin sections? Thanks-
>> Best Regards,
>> Mary Vaughan HT(ASCP)
>> Roswell Park Cancer Institute
>> Pharmacology + Therapeutics
>> Elm + Carlton Sts.  CDC-121
>> Buffalo, NY 14263
>>
>
>Mary,
> Post-fix the tissue in formalin or another LM fixative.
>Glutaraldehyde makes tissue a wee bit chromophobic.
>Ian.
>
>Dr. Ian Montgomery,
>West Medical Building,
>University of Glasgow,
>Glasgow,
>G12 8QQ,
>Scotland.
>Tel: 0141 339 8855 Extn. 6602.
>Fax: 0141 330 4100.
>e-mail: ian.montgomery@bio.gla.ac.uk
>
>
>
>
>----------------------------------------------------------------------
>
>Date: 15 Feb 1999 08:02:36 -0600
>From: Alex Brown <AlexB@nayrshire.scot.nhs.uk>
>Subject: RE: disposal
>
>Hi Pam,
> We have a waste disposal unit in our sink. It does jam
>occasionally ( when someone drops a blade or cassette down it ) but I
>wouldn't be without it..
> Alex Brown
> Kilmarnock, Scotland.
> ----------
>From: krenek@gtwn.net
>To: histonet
>Subject: disposal
>Date: 14 February 1999 19:30
>
>I would like to know if other histo departments have a disposal in the
>sink at the grossing station.  Every other hospital I have ever workd at
>has, but at my current job we  have been told that it is an infectious
>hazard and we could not get one.  It seems to me having to pick the all
>the lovely stuff out of the sink drain by hand is more of a hazard.  Is
>this something new I don't know about.  I would like to have some facts
>to take to my supervisor on this.  Thanks for the help.
>
>Pam Krenek
>
>
>
>Here are the messages received yesterday!
>
>