Re: anti-SMA

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From:John Bliss <bliss@zymed.com> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Dear Jan Willen de Groot:

Concerning IHC staining of vessel walls embedded in methacrylate: I would
recommend immunogold labeling of methacrylate-embedded tissues (see Tacha,
D. et al. J Histotech 16(1):13-17 (1993). Zymed makes HistoGold(tm) kits
which are ideal for this use. Also if you are having trouble with the SMA
antibody, you might consider anti-CD34 which reacts with hemopoietic stem
cells and small-vessel endothelial cells.

Regards,
--
Sincerely,

John Bliss
Zymed Laboratories, Inc
458 Carlton Court
So. San Francisco, CA 94080
Tel: 800-871-4494
FAX: 650-871-4499
E-mail: histo@zymed.com
Website: www.zymed.com

--
Jan Willem de Groot wrote:

> Hello Histonetters,
>
> We are interested in immunohistochemical staining of human vessel walls.
> For this reason we have some problems with anti-SMA in tissues that are
> embedded in Technovitt 8100. Is somebody familiar with anti-SMA
> immunohistochemical staining in tissues that are embedded in Technovitt
> 8100 (methacrylate)?
>
> I thank you in advanced,
>
> Greetings,
>
> Jan Willem de Groot
>
> J.W. de Groot
> Utrecht University
> Dept. of Functional Anatomy
> P.O. Box 80039
> 3508 TA Utrecht, the Netherlands
> tel.+31 30 2538323 fax +31 30 2539030
> e-mail j.degroot@fa.ruu.nl
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