Re: Nile Red protocol

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From:"J. A. Kiernan" <> (by way of histonet)
To:histonet <>
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On Wed, 17 Feb 1999 wrote:

> Hi.  Does anyone out there have a protocol for Nile Red lipid staining?
> or a reference to one?

 Assuming that you're using Nile red as a fluorochrome:

 Greenspan & Fowler 1985 J. Lipid Res. 26: 781-789.
 Greenspan, Mayer & Fowler 1985 J. Cell Biol. 100: 965-973.
 Fowler & Greenspan 1985 J. Histochem. Cytochem. 33: 833-836.

 The second two (the 3rd especially) will probably be the
 most helpful to you.

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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