Re: CMV in-situ

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From:John Bliss <> (by way of histonet)
To:histonet <>
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Dear Dr. Margraf:

Zymed Laboratories distributes Kreatech Diagnostics' ISH products in North
America. These products include Kreatech's well regarded series of
REMBRANDT(r) ISH kits. There are four REMBRANDT kits available for CMV that
offer you a choice of digoxigenin or biotin labeled probes, and AP/BCIP or
HRP/AEC detection systems. Separate CMV probe and control slides are also
available. Please contact us and we will be glad to send you more
information. Thank you.


John Bliss
Zymed Laboratories, Inc
458 Carlton Court
So. San Francisco, CA 94080
Tel: 800-871-4494
FAX: 650-871-4499


> Dear Histonetters:
> We are having trouble with getting cytomegalovirus in-situ hybridization
>up and running in our lab and are looking for some tips from anyone who
>has  been successful  with this procedure. We are trying to use the
>Novacastro probe (biotin -labeled against  CMV early transcript RNA) and
>their kit with their recommended procedure. We have contacted them and
>tried all their suggestions to no avail. We have good positive  control
>tissue and have no problem with the multiple other viral in-situ we do in
>our lab. Any suggestions, including any commercially available probes that
>work well for you would be welcome.
> Thanks,
>  Linda Margraf, MD
> Histonet administrator
> and Associate Professor of Pathology
> University of Texas Southwestern Medical School


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