CMV in-situ
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From: | Paul Kwan <paul.kwan@es.nemc.org> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hi,
I have been quite successful with Kreatech's CMV ISH kit, now being
distributed by Zymed.
I use the digoxigenin labelled probe.
Instead of digesting with pepsin as suggested by the manufacturer, I use
protease K (100 microgram per ml protease K type XXVIII, Sigma P-4914) for 10
minutes at 37 degrees C.
Good luck,
Paul Kwan, Ph.D.
New England Medical Center
Boston
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