CMV in-situ

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From:Paul Kwan <> (by way of histonet)
To:histonet <>
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I have been quite successful with Kreatech's CMV ISH kit, now being
distributed by Zymed.

I use the digoxigenin labelled probe.

Instead of digesting with pepsin as suggested by the manufacturer, I use
protease K (100 microgram per ml protease K type XXVIII, Sigma P-4914) for 10
minutes at 37 degrees C.

Good luck,

Paul Kwan, Ph.D.
New England Medical Center

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