We have consistently had difficulty with the Alcian Blue with and without
Hyaluronidase Stain.  We are using a section of cartilege as our positive
control. We use a Phosphate buffer solution at pH 5.6 with testicular
hyaluronidase purchased from Sigma Chemical.  We use 25 mg of
hyaluronidase to 50 cc of buffer. We are using a 3% solution of Alcian
Blue ph 2.5.  Any help out there????