Trichromes

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From:Rita <angelrj@email.uc.edu> (by way of histonet)
To:histonet <histonet@magicnet.net>
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Dear histonetters,

	Our lab has been having a problem lately with our Masson's Trichrome.
We are usually staining mouse hearts.  Our muscle has been staining
purple instead of red.  We have tried different times of differentiating
in acetic acid & length of time in Bouin's.  All our solutions are made
fresh or used within 3 months time.  This has also happened with our
Gomori's Trichrome and we were purchasing the Gomori's stain pre-made.
It seems to work correctly at times, but it is very inconsistent.  Any
suggestions would be appreciated.  Thank You.

Rita Angel




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