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From:Gayle Callis <> (by way of histonet)
To:histonet <>
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Yes, but sectioning qualities differ, also staining.  I found
glut fixed tissues tended to be a tidge friable, but a skilled tech (you!)
can deal with this.  Staining intensities also change, with
H&E, so test a slide or two, tweak accordingly.  I seem to remember
more intense eosin.
One of my all time favorite fixatives was Trumps glut/formalin combo, for
a project on sheep (huge amounts of innards) that had to be paraffin,
plastic and EM processed.  This fixative gave excellent results in all camps
and made up in huge quantities easily.

Just a little bead of wisdom, must be Friday.

Gayle Callis

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