Re: motor end plate stain
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of histonet) |
To: | histonet <histonet@magicnet.net> |
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On Mon, 8 Feb 1999, Sue Danielson wrote:
> Does anyone in histoland have a favorite stain for motor end plates in
> fresh-frozen skeletal muscle biopsy tissue they would be willing to share?
> Any thoughts of references/good experiences are appreciated.
The easiest way is to fix the section (formaldehyde) and than
do a histochemical stain for acetylcholinesterase activity.
If you also want to see the innervating axons, counterstain
by a silver method - Winkelmann's is a simple one. The combined
stain looks best with thickish sections: 50-100 um. If your
requirement is simply to see where end-plates are in thinner
sections, the acetylcholinesterase method alone should be
enough. See also J. Histotechnol. Vol 19 No. 3, Sept. 1996.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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