Re: electron microscope

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From:Gayle Jensen <> (by way of histonet)
To:histonet <>
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   Yes, it is easy to do.  The quality of the micrographs depends upon the
initial fixation of the tissue.  We routinely use Carson formalin for both
light and EM.  We would rather not use the paraffin tissue but sometimes we
have no choice.  I cheat and cut the tissue into small blocks and melt it in
a 60 degree oven.   Then I do a real no, no and put the tissue into a
stoppered glass vial with xylene and put it back into the oven for 1/2 hour.
(This has worked for 28 years.)  I change the xylene at least twice.  I then
rehydrate beginning with a couple of short changes of ETOH, followed by a
few rinses of 80% ETOH, 50% ETOH and distilled water.  I then embed the
tissue as usual on a Lynx Processor.  I understand that some people mix
xylene and osmium to eliminate the rehydration steps.  I tried it several
times but had no success.  My tissue was full of holes.  Good luck.  If you
have any questions, please write.
 Gayle Jensen
 Buffalo, NY

-----Original Message-----
From: Dr. Naseem <>
To: HistoNet <>
Date: Wednesday, February 03, 1999 11:04 AM
Subject: electron microscope

>Can anyone tell me about the issue sriking my mind ..
>Can Electron microscopy be done on formalin fixed paraffin emebedded

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